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Oregon State research shows hemp compounds prevent coronavirus from entering human cells

Published September 30, 2022 by tindertender

January 10, 2022

https://today.oregonstate.edu/news/oregon-state-research-shows-hemp-compounds-prevent-coronavirus-entering-human-cells

CORVALLIS, Ore. – Hemp compounds identified by Oregon State University research via a chemical screening technique invented at OSU show the ability to prevent the virus that causes COVID-19 from entering human cells.

Findings of the study led by Richard van Breemen, a researcher with Oregon State’s Global Hemp Innovation Center, College of Pharmacy and Linus Pauling Institute, were published today in the Journal of Natural Products.

Hemp, known scientifically as Cannabis sativa, is a source of fiber, food and animal feed, and multiple hemp extracts and compounds are added to cosmetics, body lotions, dietary supplements and food, van Breemen said.

Van Breemen and collaborators, including scientists at Oregon Health & Science University, found that a pair of cannabinoid acids bind to the SARS-CoV-2 spike protein, blocking a critical step in the process the virus uses to infect people.

The compounds are cannabigerolic acid, or CBGA, and cannabidiolic acid, CBDA, and the spike protein is the same drug target used in COVID-19 vaccines and antibody therapy. A drug target is any molecule critical to the process a disease follows, meaning its disruption can thwart infection or disease progression.

“These cannabinoid acids are abundant in hemp and in many hemp extracts,” van Breemen said. “They are not controlled substances like THC, the psychoactive ingredient in marijuana, and have a good safety profile in humans. And our research showed the hemp compounds were equally effective against variants of SARS-CoV-2, including variant B.1.1.7, which was first detected in the United Kingdom, and variant B.1.351, first detected in South Africa.”

Those two variants are also known the alpha and beta variant, respectively.

Characterized by crown-like protrusions on its outer surface, SARS-CoV-2 features RNA strands that encode its four main structural proteins – spike, envelope, membrane and nucleocapsid – as well as 16 nonstructural proteins and several “accessory” proteins, van Breemen said.

“Any part of the infection and replication cycle is a potential target for antiviral intervention, and the connection of the spike protein’s receptor binding domain to the human cell surface receptor ACE2 is a critical step in that cycle,” he said. “That means cell entry inhibitors, like the acids from hemp, could be used to prevent SARS-CoV-2 infection and also to shorten infections by preventing virus particles from infecting human cells. They bind to the spike proteins so those proteins can’t bind to the ACE2 enzyme, which is abundant on the outer membrane of endothelial cells in the lungs and other organs.”

Using compounds that block virus-receptor interaction has been helpful for patients with other viral infections, he notes, including HIV-1 and hepatitis.

Van Breemen, Ruth Muchiri of the College of Pharmacy and Linus Pauling Institute and five scientists from OHSU identified the two cannabinoid acids via a mass spectrometry-based screening technique invented in van Breemen’s laboratory. Van Breemen’s team screened a range of botanicals used as dietary supplements including red clover, wild yam, hops and three species of licorice.

An earlier paper in the Journal of the American Society for Mass Spectrometry described tailoring the novel method, affinity selection mass spectrometry, to finding drugs that would target the SARS-CoV-2 spike protein.

In the later research, lab tests showed that cannabigerolic acid and cannabidiolic acid prevented infection of human epithelial cells by the coronavirus spike protein and prevented entry of SARS-CoV-2 into cells.

“These compounds can be taken orally and have a long history of safe use in humans,” van Breemen said. “They have the potential to prevent as well as treat infection by SARS-CoV-2. CBDA and CBGA are produced by the hemp plant as precursors to CBD and CBG, which are familiar to many consumers. However, they are different from the acids and are not contained in hemp products.”

Van Breemen explains that affinity selection mass spectrometery, which he abbreviates to AS-MS, involves incubating a drug target like the SARS-CoV-2 spike protein with a mixture of possible ligands – things that might bind to it – such as a botanical extract, in this case hemp extract.

The ligand-receptor complexes are then filtered from the non-binding molecules using one of several methods.

“We identified several cannabinoid ligands and ranked them by affinity to the spike protein,” van Breemen said. “The two cannabinoids with the highest affinities for the spike protein were CBDA and CGBA, and they were confirmed to block infection.

“One of the primary concerns in the pandemic is the spread of variants, of which there are many, and B.1.1.7 and B.1.351 are among the most widespread and concerning,” he added. “These variants are well known for evading antibodies against early lineage SARS-CoV-2, which is obviously concerning given that current vaccination strategies rely on the early lineage spike protein as an antigen. Our data show CBDA and CBGA are effective against the two variants we looked at, and we hope that trend will extend to other existing and future variants.”

Van Breemen said resistant variants could still arise amid widespread use of cannabinoids but that the combination of vaccination and CBDA/CBGA treatment should make for a much more challenging environment for SARS-CoV-2.

“Our earlier research reported on the discovery of another compound, one from licorice, that binds to the spike protein too,” he said. “However, we did not test that compound, licochalcone A, for activity against the live virus yet. We need new funding for that.”

Timothy Bates, Jules Weinstein, Hans Leier, Scotland Farley and Fikadu Tafesse of OHSU also contributed to the cannabinoid study.

About the OSU College of Pharmacy: The College of Pharmacy prepares students of today to be the pharmacy practitioners and pharmaceutical sciences researchers of tomorrow by contributing to improved health, advancing patient care and the discovery and understanding of medicines.

STORY BY: 

Steve Lundeberg, 541-737-4039
steve.lundeberg@oregonstate.edu

SOURCE: 

Richard van Breemen, 541-737-5078
Richard.vanbreemen@oregonstate.edu

https://oregonstate.edu/digital-millennium-copyright-act

Endocannabinoid Sytem

Published August 24, 2022 by tindertender

The Endocannabinoid system’s primary function is overall bodily homeostasis. So it comes with no shock that [They] have gone out of there way to sabotage this entire body/plant relationship with propaganda for hundreds of years.

Your body. Which is substantially older than your Government came intact from God’s design with an endocannabinoid system. Hemp is not psychoactive… And yes, livestock thrived on it w/o the use of chemicals/pharmaceuticals. Our ancestors were smart, trust that. Times are changing.

How much CBD should you take? https://www.verywellmind.com/cbd-dosages-how-much-cbd-should-you-take-5078580

Do you remember #Maj12 tweets? They shared some interesting tweets for awhile and put out this info on CBD and Cannabis. screenshots from Majestic Disclosure – go.shr.lc/3dywQYj The big file with everything is at the link.

https://doc-0s-a4-docs.googleusercontent.com/docs/securesc/5llid35n1rdqd0uqai87a3h7kul5uj1b/oili5d7337dv3tbln8n3ak8mau4ir30t/1661367075000/09388410764549032989/14599241853801362017/1Jn6fFWeafvoRyjWhp8SflGg6CNFxuID-?e=download&ax=AI9vYm4_RewUCIncQf0yusH-0BhhBZ5vt6kgsnulPmXeo7P2y-UntCokz9Wb4O51G-MSt_vYiwfX0liTfoIuapQY9LHGbW2VwgPifwnspojf5ZyRgB1gy3C1zrvQ-0WjWy8FmzA9pPpFb8EDP-_BoOpHPppQNNbk2tAsPG_Ca-08SRfMdwc2wdDwV0sTJdDUUW2IfkyYeY1xNqdULC8u2H14i4TO_t-Dv6hnV1amGzAeKMgSDyYDkBRsXGQzUgtFLo3Pfoq28A4RqAWnZ6P8vWPRpTiaPNUOpkzy-CcTiLZ8FFKJqTeDhXhkL1xQGB7V0iDDgvOegLONXCgiREI17eMnki-UT-5G06zoeUnLIRtE42SaBp_S2DqQGBRVnWj9tbOHcldLEilTcQjW6UGu-JT-DSn5OCr9WVJIw3ZHj1xzJnBJ_DMItaLhSAGNW_ozkaNXC3u2zfIVixY4BDVc85Nz8jhO1Oz-a25qctmM5413VTpU5gQCfwAZefHb6joThOqgC0T–_p5mWUoNiunsG29RPm0hIM_BEXRUXiO2HmY6krIQZ94-XpcqJjqyT7iLQNN1SA-2Hh1M_GvCAhRo6qfXgYFmRSR_ngtFUj37n0-wmNT0lVgdxiO3LYXf-yHrMFpJo8wcMOnpuAmcLtacxPQD-nR_V5i4kKlVudxGUtRugfe6aSujGJbeiQyWqwbuBkvEzatd2ByCuCExA&uuid=07b62ada-eeac-4c40-be6b-58bc1e6d6968&authuser=0

Releasing Disfunction

Published July 3, 2022 by tindertender

Each person has methods used, tools, to inspire them, to give cause for thought expansion.

Some like to judge these methods, these tools. They tell you you shouldn’t use them. That the only useful information comes from the silence.

It’s good people are so firm in their beliefs.

Now if they would allow others to remain firm in their beliefs also.

It’s called honoring another’s path without judgement.

I can see the need to be critical if it causes harm to self or others.

This is usually not the case.

Usually it’s simply an emotional status of one trying to force another to see things their way …. then you get two emotional statuses trying to make the other see things their way. Then others will choose sides.

It is time to put away childish behaviors.

Division is a sickness, fostered by woundedness.

Healing is so important. But it requires effort. It requires pulling apart everything we believe about self, evaluating it and making adjustments as “self” determines necessary …. not an other.

Sharing is caring.
If it is done respectfully.

The Healer

Published March 28, 2022 by tindertender

A poem that I wrote for my beautiful friend, but it fits many of the women that were at our Corn Ceremony last weekend, and many more that I know. We missed you. 

With love,

~ Darlene V. Terry

She walks the mountains, valleys

Rivers, and creeks

Verdant meadows

Dusty, dry, desert gulches

Seeking, searching

With eyes sharp and quick

For the hidden magic

Behind, below, within

Heart shaped leaves

Round globes, medicinal sticks

Absorbing the knowing

Formulating formulas

Tinctures, salves, teas

Compresses, tricks

Bending the magic to her will

Healing wounds

Healing spirits, coughs, colds

Cuts, and nicks

The healing circling

In and around and through

Air and water, fire and stone

Love and song

Returning to heal her heart

Warm mystic, sacred

Powerful, and strong

Medicine woman

Hydroxychloroquine Testimonies

Published July 30, 2020 by tindertender

People who have been treated, or know someone who’s been treated are starting to speak out. First hand experience cannot be refuted.

https://www.lifesitenews.com/mobile/opinion/this-indian-slum-contained-a-possible-covid-19-disaster-with-hydroxychloroquine?__twitter_impression=true

https://www.newsweek.com/key-defeating-covid-19-already-exists-we-need-start-using-it-opinion-1519535

Visiting The Ocean

Published July 25, 2020 by tindertender

This morning I decided to visit the coast line, and oh how happy I am that I made that choice!

It was quite busy! I guess everyone had cabin fever, lol.

The children were so beautiful as they ran and played in the ocean. One brought the remnants of a tiny crab to his father who told him to drop it. haha

There were flowers everywhere!

This lily was actually HUGE

Apparently, the chickens have hatched, and they are dancing.

I was visited by Dragonfly. He kept flying around me. I took many photos, however these two are the only ones that weren’t blurry. What a beauty!

Of course, what is a day at the beach without ice cream?

On the road again … viewpoints!

Took a curvey route back to the city. Stopped to look at a creek and another friend said hello!

I drove all day with the windows down, the wind blowing my hair around, refreshing my energy body, blessing me in a way incomparable.

Aaaahhhhhh …. relaxation and happiness in the fullest.

Thank you GAIA. I love you so much!

PS ~ I have a bag of sand.

Current Truth About Society

Published June 22, 2020 by tindertender

A society that keeps cures a secret so they can continue to sell medication for profit is not a true society, but a mental assylum.

So much beneficial technology and ways of being have been denied the people, all for profit and power.

It is time to flip this.

Photo: https://unsplash.com/@zacdurant
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